Design a primer on gene construction kit
TCATTTCTGGGATCCTGATACAAATAATAATTTCTCAAAGGATAATAGTTGGTATTTAGCTTATTCTATAĬCTGACACAGGGGAATCACAAATAAGAAAATTTTCAGCATTAGCTAGATATGAATGGCAAAGAGGAAATTĪTAAACAAGCTACATTCTATCTTGGAGAAGCTATGCACTATTTTGGAGATATAGATACTCCATATCATCC GCGAGTGTTAATCGTTATCAAAAAGGGGAGATTAATACTTGAAAAAAATTAACGGGGGATATAAAAATGAĪAAGAAAGATTTGTAAGGCGCTTGTTTGTGCCACGCTAGTAACTAGCCTATGGGCTGGGGTATCAACTAAĪGTCTACGCTTGGGATGGAAAAATTGATGGAACAGGAACTCATGCTATGATTGTAACTCAAGGTGTTTCAĪTCTTAGAAAATGATATGTCCAAAAATGAACCAGAAAGTGTAAGAAAAAACTTAGAGATTTTAAAAGATAĪCATGCATGAGCTTCAATTAGGTTCTACTTATCCAGATTATGATAAGAATGCATATGATCTATATCAAGA TATTAAAAAATATTTAAAAAATATTCAAAAGTTTAGTGAGGTTATGTTAATTATATGGTATAATTTCAAT TTCATATTAAAAATAAGTTTAATAATTTAGAGTGGGTGAGTTTAGATATTTTAAATTAAAATTTGAATTT TTATGAGTAAAAATTCTTATTAAATTAAAAAACAATATTTAACTTATTATAACACTAATAATTGTAAATT TTTGTTCACTAAAGAATATATTTGGAAGAGTTAAATAAGTATTTTCTAGGTTAAAACCTGTTTTTGATTGĪAAATTTTTATTATCCATATTAAAATCCTTTGCCTTATAATTTATTTCAAATTTTATTCCATCCCTTATA TTAAGCCCAAGTTCTTTAGCAAGAGAAGTATTAAACTTAATAAGTTTAGGATTTTTTGAACCCTTTGGAT GGTAATACCTGGGAAAGTTTCATTTCCTGCAAATATATTAAGTCCAAAATCGCTGTTTAAAATTTCTTCA
![design a primer on gene construction kit design a primer on gene construction kit](https://www.takarabio.com/images/200-Cloning/CC-TechFigs/InFusionMutagenesis_01_2719_T.gif)
TAAGCAAAAGTTCCAACTCTAATGTGACTAGAAGCTATTCTTGTTAATATAGCACCTTGTTCAAATTTTTĬTCTTAAAACTTCCTCACCAGTACTTACTACAGCAAGGCTTCTAGTTGTAGGAATTCCAAGACTATGCATĪCCTTCACTTATTATATATTCTCTAAGCATAGGTGCAAGGGCAGCCTTTCCATCTCCACCTCTTGAATATĪTTGTTCTACCAGACCCTTTTAATTGAACATCATATCTTTTACCATCTTTAGTTACATGTTCTCCTAATAĪGAGTGCTCTACCATCTCCTAGCATTGTAAAATGGCCAAATTGGTGACCGGCATAAGCCTGTGCAATTGG TAAGTTCAGCTTGACGATTTATTACCTCTTCAAGAAATAAAATATATTTATTTTCACTATTAGCTATATTĪGGAAAGTGTCTTTTAATAGTGTAGTCAGCAAGACTTTTAAGATCCTCTAAAGTACCCCATTGAGCTGCA TCTCCTGAGATTACCATATTATCAGTGTTCATAACCCCATGAATGAATCCAACACTTTGCCACTTAACTA
![design a primer on gene construction kit design a primer on gene construction kit](https://international.neb.com/-/media/nebus/page-images/products/dna-modifying-enzymes-and-cloning-tech/goldengate_correctassembly.png)
I have a sequence for C.perfringens alpha toxin, i am suppose to design primers with his-tag for cloning in pET28+ vector.Am new to molecular biology and really dont know where to begin.Anyone who can help please?ĪAGCTTGATATGGACTTTTTAGCAATATTAACAGCCTCATCTAGGTTAGGATTTAGAAGAGGAAGTAGTGĬTTCTGAGAATCTAGCTAAGTTCCATAAGGCCATATTAGGTTGATTTCCATAGGCATATCTACCAGCATAĪTCAATGGAACTAAATACTGTGTTTGTATCATAAGTATCCATAAATGCACATGGTCCATAATCTATAGTT I would like anyone to give me a detailed answer to my question if possible.